Journal: Stem Cell Reviews and Reports

Article Title: The Treasury of Wharton's Jelly

doi: 10.1007/s12015-021-10217-8

Figure Lengend Snippet: Evaluation of ecto-, endo-and mesodermal differentiation of UC-MSCs spheroids. To evaluate the differentiability into three different germ UC tissue was let grown out on chitosan coated surfaces until UC-MP cells performed spheroids from adhering cells. For multiplication spheroids with a diameter between 80–100 μm or 100–200 μm were seeded on collagen I coated surface until confluence was reached. Adherent cells were trypsinized and seeded onto matrigel coated surface for tri-lineage-differentiation. Phase contrast microscopy images demonstrated the morphology of cells after 1, 4 and 7 days. ( a ) The scale bars represents 50 μm. For confirmation of early differentiation into three germ layers, cells were with stained with appropriate ecto- endo- and mesodermal specific marker Pax6, Sox-2, and Sox-17, CD184 and CD140, CD144b, ( b ). The scale bars represent 100 μm

Article Snippet: The medium was changed daily, and the cell morphology was detected by phase contrast microscopy (Axiovertt 40 CFL, Carl Zeiss Microscopy, Munich, Germany).

Techniques: Microscopy, Staining, Marker